SEQUENTIAL MASS SPECTORMETRY (MSⁿ)

GT utilizes pioneering ion trap sequential mass spectrometry (MSⁿ) methodology to identify and characterize isomeric glycan structures. Analysis can be performed on most glycoconjugates, including but not limited to N-linked glycoproteins, O-linked glycoproteins, glycolipids, and glycosaminoglycans. The GT MSⁿ sequencing methodologies resolve major problems in current industry-standard glycoanalysis:

• Heterogeneity: Because individual glycoproteins and glycosylation sites carry many different oligosaccharide structures, the glycosylation of a protein is almost always heterogeneous.
• Branching: The non-linear structure of carbohydrates increases analytical difficulty.
• Isomeric oligosaccharide structures: The branched structure and identical molecular weight of many monosaccharides result in many different oligosaccharide structures with the same molecular weight, greatly complicating glycosylation analysis.
• Multi-method analysis: Current methodologies of glycoprotein analysis (chromatography, GC-MS, enzymology, MS profiling, and Tandem MS/MS) cannot perform de novo analysis of glycans, and combining these methods has not overcome the individual methodologies' intrinsic limitations.

GT MSⁿ, utilizing sequential rounds of product ion isolation and fragmentation, provides a superior method that can extract sufficient information for the accurate characterization of glycans. GT MSⁿ methodology can be employed independently or in conjunction with your current glycan analysis methods.